A Novel 3D In Vitro Model of the Human Gut Microbiota

Published in Scientific Reports, vol. 10 (2020) · Nature Portfolio · Open access, CC BY
DOI: 10.1038/s41598-020-78591-w

Summary

Studying the gut microbiota in living subjects is hampered by the difficulty of reaching the gut, the near-impossibility of continuous monitoring, and ethical constraints on animal work. This study presents a three-dimensional in vitro model in which the microbial community grows on an electrospun scaffold - accessible, observable, and reproducible. Being able to watch how microbes interact with each other and with the host environment, rather than inferring it from stool samples, is what such a platform makes possible.

Authors & Affiliations

  • Francesco Biagini
    Research Centre "E. Piaggio", University of Pisa, Italy
  • Marco Calvigioni
    Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Italy
  • Anna Lapomarda
    Research Centre "E. Piaggio", University of Pisa, Italy
  • Alessandra Vecchione
    Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Italy
  • Chiara Magliaro
    Research Centre "E. Piaggio", University of Pisa, Italy
  • Carmelo De Maria
    Research Centre "E. Piaggio", University of Pisa, Italy

Linari Electrospinning Systems

Linari Engineering electrospinning system
The scaffolds were spun for one hour on a Linari Engineering system at 30 kV, with a feed rate of 1.2 ml/h and a needle-to-collector distance of 10 cm - the same parameters this group used in their subsequent work on microbiota adhesion. See our range of systems.

Topics

Gut Microbiota 3D In Vitro Models Alternatives to Animal Testing Microbial Communities

Materials

Electrospun polymer scaffolds
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