Summary
Chronic, non-healing wounds are difficult to treat, and growth factors that stimulate skin regeneration are a promising but fragile therapeutic tool. This study develops an electrospun nanomaterial that carries blood-derived growth factors for dermal wound healing, using platelet-rich plasma (PRP) as a natural, patient-compatible source of those factors. Smooth chitosan/poly(ethylene oxide) (PEO) nanofibres were produced by electrospinning and loaded with PRP, and the mats kept their structure under moist conditions while preserving PRP bioactivity. The authors first confirmed that PRP stimulates keratinocyte and fibroblast growth in vitro, then showed that the PRP-loaded nanofibrous support influenced the growth, metabolic activity and morphology of these key skin cells. The results point to a synergistic effect between fibre nanotopography and incorporated growth factors, supporting the mats as a candidate delivery system for wound regeneration.