Electrospun Structures Made of a Hydrolyzed Keratin-Based Biomaterial for In Vitro Tissue Models

Published in Frontiers in Bioengineering and Biotechnology, vol. 7, 174 (2019) · Frontiers Media · Open access, CC BY
DOI: 10.3389/fbioe.2019.00174

Summary

Keratin extracted from poultry feathers - a waste stream of considerable volume - is characterised here in hydrolysed form and processed by electrospinning into structures for in vitro tissue models. The biomaterial was blended with type A porcine gelatin and cross-linked with GPTMS, first cast as films and then spun into fibres, with applied potential, flow rate and other parameters varied systematically. Comparing the two forms shows what the fibrous architecture adds beyond the material itself.

Authors & Affiliations

  • Gabriele Maria Fortunato
    Research Centre "E. Piaggio", University of Pisa, Italy
  • Francesco Da Ros
    Department of Molecular Medicine, University of Padua, Italy
  • Sara Bisconti
    Research Centre "E. Piaggio", University of Pisa, Italy
  • Aurora De Acutis
    Research Centre "E. Piaggio", University of Pisa, Italy
  • Francesco Biagini
    Research Centre "E. Piaggio", University of Pisa, Italy
  • Anna Lapomarda
    Research Centre "E. Piaggio", University of Pisa, Italy

Linari Electrospinning Systems

Linari Engineering electrospinning system
The structures were produced on a Linari Engineering system (Pisa, Italy), varying the applied potential, the flow rate and the other process parameters to map the working window for this biomaterial. See our range of systems.

Topics

Waste Valorization In Vitro Tissue Models Cross-linking Process Parameter Mapping

Materials

Hydrolyzed keratin Porcine gelatin GPTMS
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