Summary
Adipose-derived stem cells are an accessible cell source for repairing soft tissue injury, but what governs their effectiveness at the molecular level is not well understood. This study uses single-cell multiomics together with a 3D model to examine the role of the protein IGFBP3 in those cells, comparing cell populations in a mouse injury model. Electrospun polycaprolactone provides the three-dimensional environment in which the cells are assessed - the scaffold being what makes the multiomic comparison physiologically meaningful rather than a flat-culture artefact.
Authors & Affiliations
Linari Electrospinning Systems
Linari Engineering electrospinning system
The polycaprolactone scaffolds were produced on a Linari Engineering system, with the solution fed continuously at a constant flow rate by syringe pump. The paper does not name the model; see our range of electrospinning systems.