Screening of Electrospun PS/PCL Scaffolds for 3D Triple-Negative Breast Cancer Models

Published in Scientific Reports (2025) · Nature Portfolio · Open access, CC BY-NC-ND
DOI: 10.1038/s41598-025-05871-8

Summary

Triple-negative breast cancer is aggressive and lacks targeted therapies, and studying it properly requires culture systems where cells interact with an extracellular matrix rather than a flat plastic surface. This study screens electrospun polystyrene/polycaprolactone scaffolds for that role, systematically varying three things: the orientation of the electrospinning setup, the solvent, and the polymer composition. Treating the machine configuration itself as an experimental variable is unusual, and it shows how directly the setup governs the scaffold a tumour model needs.

Authors & Affiliations

  • Sara Ausellé-Bosch
    TargetsLab - Oncology Unit, University of Girona, Spain
  • Marta Pardo
    TargetsLab - Oncology Unit, University of Girona, Spain
  • Marta Pareja
    TargetsLab - Oncology Unit, University of Girona, Spain
  • Emma Polonio-Alcalá
    Precision Oncology Group (OncoGIR-Pro), Girona Biomedical Research Institute, Spain
  • Teresa Puig Corresponding
    TargetsLab - Oncology Unit, University of Girona, Spain

Linari Electrospinning Systems

Linari Engineering electrospinning system
The scaffolds were produced on a Linari Engineering system (Pisa, Italy), with parameters chosen to form a stable Taylor cone throughout the process. The paper does not name the model; see our range of electrospinning systems, which support both horizontal and vertical configurations.

Topics

3D Tumor Models Triple-Negative Breast Cancer Setup Orientation Scaffold Screening

Materials

Polystyrene Polycaprolactone
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