Study of the Adhesion of the Human Gut Microbiota on Electrospun Structures

Published in Bioengineering, vol. 9(3), 96 (2022) · MDPI · Open access, CC BY
DOI: 10.3390/bioengineering9030096

Summary

Bacterial adhesion to surfaces is well studied, but nearly always for a single species and nearly always to prove a material is antimicrobial. This study inverts the question: it asks how an entire microbial community - the human gut microbiota - colonises a surface and forms stable biofilms. Two electrospun structures made from natural polymers serve as the substrates, so the aim is not to prevent adhesion but to support it faithfully enough to study a complex ecosystem in vitro.

Authors & Affiliations

  • Francesco Biagini
    Research Centre "E. Piaggio", University of Pisa, Italy
  • Marco Calvigioni
    Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Italy
  • Carmelo De Maria
    Research Centre "E. Piaggio", University of Pisa, Italy
  • Chiara Magliaro
    Research Centre "E. Piaggio", University of Pisa, Italy
  • Francesca Montemurro
    Research Centre "E. Piaggio", University of Pisa, Italy
  • Diletta Mazzantini
    Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Italy

Linari Electrospinning Systems

Linari Engineering electrospinning system
The structures were spun for one hour on a Linari Engineering system at 30 kV with a feed rate of 1.2 ml/h. Sustained runs at high voltage are where a stable generator matters - see our range of systems.

Topics

Gut Microbiota Biofilm Formation Bacterial Adhesion In Vitro Models

Materials

Natural polymer scaffolds
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