Effects of Electrospinning on the Viability of Ten Species of Lactic Acid Bacteria

Published in Pharmaceutics, vol. 11(9), 483 (2019) · MDPI · Open access, CC BY
DOI: 10.3390/pharmaceutics11090483

Summary

Probiotic bacteria have to survive whatever process encapsulates them, and electrospinning subjects them to high voltage, shear and rapid solvent evaporation - which is why spinning live organisms has barely been attempted. This study puts the question to the test across ten species of lactic acid bacteria, examining fibre morphology alongside bacterial viability after spinning. Establishing which species survive, and under what conditions, is the groundwork for using nanofibres as a probiotic delivery system.

Authors & Affiliations

  • Špela Zupančič Corresponding
    Faculty of Pharmacy, University of Ljubljana, Slovenia
  • Katarina Škrlec
    Department of Biotechnology, Jožef Stefan Institute, Ljubljana, Slovenia
  • Petra Kocbek
    Faculty of Pharmacy, University of Ljubljana, Slovenia
  • Julijana Kristl
    Faculty of Pharmacy, University of Ljubljana, Slovenia
  • Aleš Berlec
    Department of Biotechnology, Jožef Stefan Institute, Ljubljana, Slovenia

Linari Electrospinning Systems

Linari high voltage power supply HVG-P60-R-EU
The fibres were spun on a Linari Engineering system (Valpiana, Italy) with the high voltage power supply model HVG-P60-R-EU - our 60 kV positive-polarity generator in the European configuration. See the high voltage generator page; the current control unit is coded HVG-CONT-LCD.

Topics

Probiotic Encapsulation Bacterial Viability Live Cell Electrospinning Delivery Systems

Materials

Lactic acid bacteria Polymer nanofibers
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