Engineered Nanofiber-Hydrogel Systems for Colorimetric Lactate Sensing from Breath

Summary

Diagnosing chronic airway inflammation such as asthma normally requires elaborate procedures and trained clinicians. This work proposes a far simpler route: capturing lactate directly from exhaled breath on an engineered nanofibre mat and reading the result by colour change. The fibres are made of positively charged nylon-poly(allylamine hydrochloride) and were optimised for mat thickness, additive content and lactate capture efficiency, binding the analyte electrostatically so that the amount retained reflects its concentration in breath aerosol. Combined with an enzyme-entrapping hydrogel, the system delivers non-invasive sampling followed by colorimetric detection, pointing towards a wearable, point-of-care format for respiratory monitoring.

Authors & Affiliations

• Barbara V. Grotz

  Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Germany
• Konstantin Rogalla von Bieberstein

  Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Germany
• Nongnoot Wongkaew

  Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Germany
• Axel Duerkop

  Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Germany
• Margaret W. Frey

  Department of Human Centered Design, College of Human Ecology, Cornell University, Ithaca, NY, USA
• Antje J. Baeumner Corresponding

  Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Germany

Linari Electrospinning Systems

Topics

Materials