Summary
Testing whether a drug crosses the blood-brain barrier normally requires animal brains, with all the ethical and practical limits that implies. In vitro models offer a closed system in which the relevant variables can be set and monitored instead, and this study builds one on porous cellulose acetate scaffolds. The scaffolds are optically transparent, which is the decisive property: it allows the cell layer to be observed directly under the microscope while the barrier function is being measured, making the model suitable for high-throughput quantitative screening.
Authors & Affiliations
Linari Electrospinning Systems
Linari high voltage generator (dual connection)
A Linari Engineering generator was connected to both the syringe needle and the collector at an applied voltage of 12 kV - the double-generator configuration that becomes possible when the collector is insulated from ground, as on our high voltage systems (control unit HVG-CONT-LCD).